Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
نویسندگان
چکیده
منابع مشابه
Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
BACKGROUND Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing (CBP) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol. RESULTS The Aspergillus tubing...
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Shwanniomyces occidentalis possess highly efficient alpha amylase activity that enable this yeast to use raw starch as an carbon source. S.occidentalis alpha amylase gene was cloned into the Saccharomyces cerevisiae under the control of different powerful constitutive promoters, ADH1, GPD and TEF. So that the recombinant strains which carries the same gene under the control of different promote...
متن کاملInfectious keratitis caused by Aspergillus tubingensis.
PURPOSE To report 2 cases of keratomycosis caused by Aspergillus tubingensis. METHODS The therapeutic courses were recorded for 2 male patients, 52 and 78 years old, with fungal keratitis caused by black Aspergillus strains. Morphological examination of the isolates was carried out on malt extract agar plates. A segment of the beta-tubulin gene was used for molecular identification. Antifunga...
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In red-white sectored colonies of Saccharomyces cerevisiae, derived from mitotic cells grown to stationary phase and irradiated with a light dose of x-rays, all of the segregational products of gene conversion and crossing over can be ascertained. Approximately 80% of convertants are induced in G1, the remaining 20% in G2. Crossing over, in the amount of 20%, is found among G1 convertants but m...
متن کاملEnhanced Gene Conversion and Postmeiotic Saccharomyces Cerevisiae
Previous study has demonstrated that incubation of yeast cells of strain AP1 in sporulation medium at 36" permits them to begin meiosis but that they become arrested at pachytene and undergo enhanced intragenic recombination between ade2 heteroalleles. Tetrad analysis was undertaken to characterize the altered program of meiotic recombination more widely. In one set of experiments, pachytene-ar...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2013
ISSN: 1754-6834
DOI: 10.1186/1754-6834-6-167